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Olfactory glial cell review

Olfactory mucosa, stem cells, olfactory ensheathing cells, cell therapy

Teaser:
Olfactory mucosa, the sense organ of smell, is an adult tissue that is regenerated and repaired throughout life to maintain the integrity of the sense of smell. Olfactory receptor neurons arise from stem cells and their axons grow from the ...

 

Custodian:
• Dr James St John

Contributors:
• James St John

Microscopes:
• Laser scanning confocal microscope - Fluoview FV1000

Registry Name:
Griffith University

Project Name:
• Olfactory glial review

 

Full record

 

Tyrosine Hydroxylase immunofluorescence in the Substantia Nigra: relevance to pathological assessment of post-mortem tissues.

Brain, substantia nigra, dopaminergic inter-neurons, multilabelling immunofluorescence, antigen retrieval, antigenecity, antigen retreaval, tyrosine hydroxylase, calbindin, calretinin, DAPI, polyethyline glycol, paraffine sections,

Teaser:
Multiple factors may affect immunofluorescence in post mortem brain, with significant implications for research and clinical investigations using human tissue.

 

Custodian:
• Dr Adrian Meedeniya

Contributors:
• Natalie Romond

Microscopes:
• Epifluorescence microscopes - Zeiss AxioImager Z1

Registry Name:
Griffith University

Project Name:
• Adult Stem Cell Transplantation Therapy in Parkinsonian Rat

 

Full record

 

Automated classification of dopaminergic neurons in the rodent brain.

Brain, substantia nigra, olfactory bulb, dopaminergic inter-neurons, multilabelling immunofluorescence, morphometry, segmentation, feature extraction, classification, Neural networks, Support Vector Machines, Back Propagation Neural Networks and Multinomial Logistic Regression

Teaser:
In addition to being important to neurobiologists, accurate segmentation is a vital component of an automated image processing system.

 

Custodian:
• Dr Adrian Meedeniya

Contributors:
• Gervase Tuxworth
• Brenton Cavanaugh

Microscopes:
• Epifluorescence microscopes - Zeiss AxioImager Z1

Registry Name:
Griffith University

Project Name:
• Adult Stem Cell Transplantation Therapy in Parkinsonian Rat

 

Full record

 

Images of the olfactory bulb of 18 month old mice

Brain, substantia nigra, olfactory bulb, dopaminergic inter-neurons, multilabelling immunofluorescence, morphometry, segmentation, feature extraction, classification, Neural networks, Support Vector Machines, Back Propagation Neural Networks and Multinomial Logistic Regression.

Teaser:
The absence of a key neurotrophin together with ageing would give rise to elevated neurodegeneration, reflective of the human condition. The degeneration relaed changes would be reflected in the morphometry os dopaminergic interneurons

 

Custodian:
• Dr Adrian Meedeniya

Contributors:
• Gervase Tuxworth

Microscopes:
• Epifluorescence microscopes - Zeiss AxioImager Z1

Registry Name:
Griffith University

Project Name:
• Adult Stem Cell Transplantation Therapy in Parkinsonian Rat

 

Full record

 

Multidimensional epifluorescence data for quantification of neurodegeneration in a rodent model

Brain, substantia Multidimensional nigra, dopaminergic epifluorescence data for neurons, astrocytes, quantification of microglia, complex 1,neurodegeneration in a neurodegeneration, Parkinson’s disease, rodent model oxidative stress,reactive oxygen species, rotenone, immunofluorescence, densitometry,

Teaser:
This Collection consists of 148 images. The media were used to obtain densitometry data on several key proteins expressed in the brain that correlates to neural function. The expression levels of these proteins were assayed in an animal model of ...

 

Custodian:
• Dr Adrian Meedeniya

Contributors:
• Adrian Meedeniya
• Anwar Norazit

Microscopes:
• Epifluorescence microscopes - Zeiss AxioImager Z1

Registry Name:
Griffith University

Project Name:
• Adult Stem Cell Transplantation Therapy in Parkinsonian Rat

 

Full record

 

Images of the Olfactory bulb of 3 month old mice

Brain, substantia nigra, olfactory bulb, dopaminergic inter-neurons, multilabelling immunofluorescence, morphometry, segmentation, feature extraction, classification, Neural networks, Support Vector Machines, Back Propagation Neural Networks and Multinomial Logistic Regression.

Teaser:
Accurate morphological characterization of the multiple neuronal classes of the brain would facilitate the elucidation of brain function and the functional changes that underlie neurological disorders such as Parkinson's diseases or Schizophrenia. This media was collected to compare the performance ...

 

Custodian:
• Dr Adrian Meedeniya

Contributors:
• Gervase Tuxworth

Microscopes:
• Epifluorescence microscopes - Zeiss AxioImager Z1

Registry Name:
Griffith University

Project Name:
• Adult Stem Cell Transplantation Therapy in Parkinsonian Rat

 

Full record

 

Thymidine analogues for tracking DNA synthesis

BrdU, EdU, neurogenesis, DNA, thymidine analogue, click chemistry, in vivo, in vitro.

Teaser:
Replicating cells undergo DNA synthesis in the highly regulated, S-phase of the cell cycle. Analogues of the pyrimidine deoxynucleoside thymidine may be inserted into replicating DNA, effectively tagging dividing cells allowing their characterisation.

 

Custodian:
• Dr Adrian Meedeniya

Contributors:
• Adrian Meedeniya
• Brenton Cavanagh

Microscopes:
• Laser scanning confocal microscope - Fluoview FV1000

Registry Name:
Griffith University

Project Name:
• The development of copper-free click chemistry to label biomolecules within living cells

 

Full record

 

Tissue sectioning for epifluorescence microscopy

Polyethyline glycol , widefield microscopy, perfusion fixation,

Teaser:
Optimising Tissue sectioning and preparation for epifluorescence microscopy. Produced a discussion paper on tissue preparation protocols and their application in multiple anatomical investigations.

 

Custodian:
• Dr Adrian Meedeniya

Contributors:
• Maria Nguyen
• Brenton Cavanagh

Microscopes:
• Epifluorescence microscopes - Zeiss AxioImager Z1

Registry Name:
Griffith University

Project Name:
• Adult Stem Cell Transplantation Therapy in Parkinsonian Rat

 

Full record

 

Fluorescent neurostereology: A novel method for quantitative analysis of midbrain dopaminergic nuclei

Brain, substantia nigra, dopaminergic inter-neurons, multilabelling immunofluorescence Stereology, absolute numbers, exclusion planes, volume estimation, optical dissector, Cavalieri principle,

Teaser:
3D image stacks acquired from four rodent substantia nigra. Collected to stereologically quantify dopaminergic neurons subclasses in the substantia nigra. Absolute numbers of the cell types are generated.

 

Custodian:
• Dr Adrian Meedeniya

Contributors:
• Adrian Meedeniya
• Brenton Cavanagh

Microscopes:
• Epifluorescence microscopes - Zeiss AxioImager Z1

Registry Name:
Griffith University

Project Name:
• Adult Stem Cell Transplantation Therapy in Parkinsonian Rat

 

Full record

 

Understanding how the multiple roles of olfactory ensheathing cells guide the growth and regeneration of olfactory axons

Olfactory mucosa, stem cells, olfactory ensheathing cells, cell therapy, transgenic,

Teaser:
The media in this collection presents a transgenic reporter mouse whose olfactory sensory neurons express ZsGreen, driven by the olfactory marker protein (OMP) promoter.

 

Custodian:
• Dr James St John

Contributors:
• James St John
• Jenny Ekberg

Microscopes:
• Laser scanning confocal microscope - Fluoview FV1000

Registry Name:
Griffith University

Project Name:
• OMP-ZsGreen mice

 

Full record